There are several Pichia GlycoSwitch® strains to choose from:
SuperMan5 SuperMan5 HIS– SuperMan5(pep4–)
SuperMan5(aox1–,Muts) SuperMan5(pep4–,prb1–) SuperMan5(pep4–,sub2–)
Strains are available from BioGrammatics and should express your target protein with a mannose-5 structure at N-linked sites. O-glycosylation may also be reduced to the mannose-5 structure. For more detailed information on Pichia GlycoSwitch®, you can download and review a presentation by Nico Callewaert or review our publication list.
Two AOX1 vectors that are compatible with both SuperMan5 strains are available from BioGrammatics.
- pJAZa – Zeocin resistance gene, AOX1, alpha mating factor
- pJAGa – G418 resistance, AOX1, alpha mating factor
One can also purchase various vectors from Life Technologies that are part of Pichia Classic for use with Pichia GlycoSwitch®. Specfically, the pPICZa and pGAPZa vectors will be compatible with the SuperMan5 strains.
GlycoSwith® vectors for transforming the SuperMan5 strains into strains making more complex human-like glycoforms are available at BioGrammatics.com
RCT has established relationships in the U.S. and Europe with the following companies to provide contract services related to Pichia GlycoSwitch®:
•BioGrammmatics (Carlsbad, CA) – In addition, to distributing and selling GlycoSwitch® strains and vectors, BioGrammatics offers custom strain development and optimization, protein production and purification, and strain transcriptome analysis. Click here to learn more.
•VTU Technology (Graz, Austria) – VTU Technology offers custom strain development and optimization, protein production and purification, and process development for scale-up. GlycoSwitch® strains from VTU Technology incorporate proprietary promoters and chaperones that significantly enhance manufacturing yields of proteins. Click here to learn more.
To order Pichia GlycoSwitch® Strains and Vectors Order Pichia GlycoSwitch
To order the pPICZ or GAPZ vectors from Life Technologies compatible with Pichia GlycoSwith® strains Life Technologies